RPI ID: 2015-038-601

Innovation Summary:
This invention introduces a non-demineralizing method for extracting proteins from bone tissue for research and diagnostics. The system uses optimized extraction buffers and a streamlined process to isolate a wider array of proteins compared to conventional techniques. It leverages hydroxyapatite chromatography and mass spectrometry to analyze the proteome directly from bone samples. By skipping the lengthy demineralization step, it preserves protein integrity and significantly reduces processing time. The method yields higher protein concentrations and offers insights into metabolic bone diseases, orthobiologics, and even ancient remains. It is ideal for developing new diagnostic assays and understanding bone-related conditions.

Challenges / Opportunities:
Conventional protein extraction from bone is time-consuming and may degrade or miss low-abundance proteins. This limits the reliability and scope of bone proteomics, affecting diagnostics and biomarker discovery. This invention improves workflow efficiency and expands protein recovery, offering new research pathways in biomedicine and paleoproteomics. It supports early detection of bone disease, quality control of bone graft materials, and retrospective analysis of fossilized specimens.

Key Benefits / Advantages:
✔ No demineralization required
✔ Faster and more complete protein extraction
✔ Preserves labile proteins for better diagnostics

Applications:
• Bone disease diagnostics
• Orthobiologic development
• Paleoproteomic research

Keywords:
#boneproteins #noninvasivetesting #orthobiologics #massspectrometry

Intellectual Property:
US Issued Patent 11,685,763 B2